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The route from Brussels to Prague is served daily by 69 bus departing from the stations of Brussels South (Gare Du Midi), Brussels-North Station, Brussels Midi / Brussel Zuid East, Gare Du Nord, Brussels North Train Station, Brussels, Nord, Bruxelles, Midi, Gare Du Midi, Brussels Airport, Brussels Gare Du Midi, Brussels Midi / Brussel Zuid West, Midi and arriving to the stations of Prague ÚAN Florenc, Prague Anděl, Prague Main Railway Station, Prague Florenc Central Bus Station, Florenc, Prague, Florenc, Prague ,Hradčanská, Prague Wilsonova Bus Station.
The average duration of the journey is 19 h 24 min. The first bus leaves at 02:00. The last is at 23:45.

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